C-peptide as a Means of Measuring Insulin

Published: 1 Peninsula NIHR clinical Research Facility, Peninsula Medical School, Exeter, UK, and clinical Chemistry, princely Devon and Exeter NHS Foundation Trust, Exeter, UK2 Peninsula NIHR Clinical Research Facility, Peninsula Medical School, Exeter, UK3 Clinical Chemistry, purple Devon and Exeter NHS Foundation Trust, Exeter, UKTitle: Stability and Re masterducibility of a Single- archetype urinary C-Peptide/Cr take inine Ratio and its Correlation with 24-h Urinary C-peptide. IntroductionThe breast is a clinical and correlation experiment to determine how passive C-peptide is in urine. For every One C-peptide produced, thither is an equal do of insulin soupcon produced in the pancreatic beta cell when pro insulin splits into insulin. at that placefrom knowing the amount of urinary C-peptide (UCP) present in a 24hrs urine attempt tells us about the proceeds dictate of insulin in the individual. C-peptide is measured rather than insulin be throw it has a extended half(prenominal) life. Materials and Methods:The research group analyzed UCP using a run-of-the-mine electrochemiluminescence immunoassay in samples obtained from 21 tidy volunteer, the stability of UCP with preservatives (boric acid, nuclear number 11 azide and sodium carbonate) were comparabilityd as come up as the reproducibility of urinary C-peptide/creatinine ratio (UCPCR) in first- and second- rashness fasting urines within a 24-h collections. My chit chat:The researchers could possess analyzed C-peptide using some some separate techniques to suss out and comp ar the racyest specificity and sensitivity of C-peptide non just curb their technique using figure electrochemiluminescence immunoassay, method actings much(prenominal) as:1. Two dimensional abate phase/reverse phase high-performance silver-tongued chromatography. 2.N- terminal sequence analysis of endoproteinase Lys_C peptide map usine routine 3.5 method on the HP G1005A N- terminal protein sequ encing system. 3.Other methods include: the ! Adaltis, RIa, the Dako Elisa, the PerkinElmer successions-resolved fluoroimmunoassay and in hold immunochemiluminometric assay4.The researchers did non summons if it was the free or bound C-peptide that was measured. ethanol is efficient to particularize and sepa step bound and free C-peptideResults:UCPCR stable at agency temperature for 24hrs at 4 degree centigrade for 72hrs2nd void fasting sample median = 0.78Second-void fasting UCPCRs were lower than first-void (median 0.78 vs 1.31, P = 0.0003) and showed less pas seul (CV 33% vs 52%), as second-void UCPCRs were not influenced by even out food-related insulin secretion. Second-void fasting UCPCR was highly correlated with 24-h UCP (r = 0.8, P = 0.00006). Study participantMy remonstrate:1.The research team could have employ a wider swan of commonwealth, because the larger the size the better the results discount be analyzed. 30 is the rule of thumb when considering sample size. A wider age field could have been use d as well, not just limiting the age between 24-54yrs. for instance monitor the C-peptide in foetus or infants female genitals show if there are differences and onward motion due to growth or whether there is an acquired or familial problem. 2.The parameters for healthy patient were not well defined for example if patients are disease free, transplant patients, or if their organs much(prenominal) as the kidneys are working properly. The races of the healthy patients were not call downed, because there might be genetic variation in regards to insulin metabolism. aliment could in any case play a feature film in insulin production, the eccentric person of food the participant eat were not mentioned or monitored. For example, patients who predominantly eat food high in carbohydrate, their blood sugar tend to rise to profuse levels, which give rise a condition known as insulin resistance, afterward attain the rate of insulin production. High levels of C-peptide also are se en with insulinomas (insulin-producing tumors) and ma! y be seen with hypokalemia, pregnancy, Cusings syndrome and renal failure. 3.The research team just now looked at the C-peptide in healthy patients. They could have compared data with those of angry patients to see the differences in C-peptide production.

Patients who are case 1 diabetic experience an autoimmune process that often starts in betimes childhood and involves the nearly complete destruction of the beta cells ( tough in the production of insulin) over time. Eventually, little or no insulin (and C-peptide) is produced. In type 2 diabetics, the patients are insulin resistant so there is a compensatory increase in insulin production and sack that merchantman also lead to beta cell damage. thus the production rate of C-peptide will vary in these both different groups of diabetic patients. 4.How the patients fasted was not well defined by the research group, the time limit of fasting can affect the production of C-peptide. also stress is another factor that can affect C-peptide production and it wasn?t mentioned in the paper. Creatine RatioMy gabfest:Different types of creatin occur in the body such as creatine monohydrate and creatine kinase (CK) also known as creatine phosphokinase (CPK). heave of CK is an extension of damage to many other diseases not only limited to problems with C-peptide. For example lowered CK can be an indication of alcoholic liver diseases which can cause fatty change, or macrovesicular steatosis. The research group did not mention the specific type of creatine they measuredIn conclusion, I don?t think the research was adequately conducted. If the research group increase the range of people they used! for the study and employed other techniques to compare specificity and sensitivity of C-peptide, as well as including sunburnt patients (type 1 and 2 diabetics) in their study, the values of the reproducibility and stability of C-peptide will subsequently be affected. Work citedhttp://www.clinchem.org/cgi/content/ scam/55/11/2035http://www.chem.agilent.com/Library/applications/59653465.pdfhttp://www3.interscience.wiley.com/journal/112468890/abstracthttp://www.clinchem.org/cgi/reprint/54/6/1023.pdf If you desire to get a full essay, rove it on our website: BestEssayCheap.com

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